Cangzhou Xingye Biotechnology Co., Ltd.

Bacillus subtilis (sales hotline:) closely related populations (referred to as the Bacillus subtilis group) are a group of Gram-positive, spore-forming bacilli with similar phenotypes. Currently, this group includes 1 valid published species. This group of bacteria is widely used in biotechnology, industry, agriculture and other fields. Therefore, it is the primary condition to achieve rapid and accurate identification and identification of strains and ensure the safety and effectiveness of their use. Based on the research on the identification of subtilis groups carried out in our laboratory, this paper summarizes and proposes the traditional classic methods based on morphological and biochemical characteristics determination and gene sequence analysis technology and specific technology based on molecular biology technology, and has carried out in practice A large number of verifications have important application value in guiding the accurate and rapid identification of this population.
Since its official name three years ago, Bacillus subtilis has been studied for many years and is widely used in industrial fermentation, agricultural production, medicine and health, food health, aquaculture and other fields. With the advancement of research technology, it was discovered that Bacillus subtilis is actually a phenotypically similar group, that is, a closely related group of Bacillus subtilis (referred to as the subtilis group). In addition to the earlier named Bacillus subtilis (Bb), Bacillus licheniformis (B.), Bacillus pumilus (B.), Bacillus amyloliquefaciens (B.), Bacillus atrophicus (Br) , the subtilis group also includes Bacillus mojave (Mojave) (B.), Bacillus death valley (Br), Bacillus sonora desert (B.), Bacillus tequila (B.), Siam Bacillus subtilis (B.) and B. subtilis are further divided into three subspecies: B.bb. b, B.bb. [] and B. b., and Bacillus amyloliquefaciens is also divided into Bb and Bb subspecies. So far, the closely related species of Bacillus subtilis have included 1 valid published species.
Since subtilis strains are closely related to human production and life, the accurate identification and identification of this type of microorganisms is very important. However, the complexity of subtilis classification brings huge difficulties to their identification. Traditional biochemical test methods for strain identification are time-consuming and labor-intensive, and the results are easily affected by environmental conditions and personnel experience. The widely used B rapid identification system, system, fatty acid analysis system and other technologies are easy to operate, fast and efficient, but for relatives The closely related subtilis group strains can only be identified to the group, but it is difficult to identify the species. Identification technology based on gene sequence analysis has gradually become an important tool for identifying strains due to its advantages of high resolution, stability and reproducibility, and specific technologies established on the basis of sequence analysis make identification faster and more targeted. In order to promote the use of new technologies and methods and improve the speed and efficiency of identification, this article reviews the research progress of traditional biochemical methods, gene sequence analysis and specific technologies in the identification of subtilis and related populations, and looks forward to its application prospects. .